University College London Zebrafish

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University College London Zebrafish

University College London Zebrafish

 

 

fish facility title

Welcome to the UCL fish facility.

Here you can find The British Zebrafish Community Line Database.

The UCL fish facility is currently comprised of four separate multi-rack systems and several stand-alone and bench top systems housed in five separate rooms. Between the systems we have a total of about 5,000 tanks – each between 1.5 litres and 10 litres. All the multi-rack systems are re-circulating systems that are fully quarantined (all lines have been established from bleached eggs).

The UCL Fish Facility was established in 1998 and if you would like to see the citation metrics for nearly all of the zebrafish papers from UCL researchers since then, you will find them here.

NEW E1/L, PILA, PILB zebrafish

  • January 10th/11th 2017
  • February 7th/8th 2017
  • March 7th/8th 2017
  • April 4th/5th 2017
  • May 9th/10th 2017
  • June 6th/7th 2017
  • July 11th/12th 2017
  • September 5th/6th 2017
  • October 3rd/4th 2017
  • October 10th/11th 2017
  • November 7th/8th 2017
  • December 5th/6th 2017

If you are a UCL group using, or considering using, zebrafish then please come and talk to us about your plans. We have no core funding for the facility and so it is crucial that all planned fish use is properly costed on your grant applications.

For any enquiries please contact the facility manager, Carole Wilson.

Click here for rules and regulations of moving fish.

CONTACT DETAILS

For general enquiries please email the facility.
To contact the Fish Facility Resource Room directly call 020 3549 5634 (internal 65634)

Carole Wilson
Head of Facility
carole.wilson@ucl.ac.uk
0203 549 5623 (internal 65623)

Heather Callaway
Operations Manager
h.callaway@ucl.ac.uk

Jenna Hakkesteeg
Senior Technician – Stock Centre
ucgajlh@ucl.ac.uk

Visila Moiche
senior technician
ana.vega@ucl.ac.uk

Paul Barwood
nursery / specialist technician
p.barwood@ucl.ac.uk

Karen Dunford
Research Assistant
k.dunford@ucl.ac.uk

Matt Wicks
Technician
m.wicks@ucl.ac.uk

Research

Our aim is to understand fundamental aspects of the structure and operation of the neural circuits that process sensory information to control animal behaviour.

We work with larval zebrafish – a vertebrate model that has a tiny, optically transparent brain – enabling us to use advanced light microscopy techniques to monitor neural activity during behaviour.

2-photon and light sheet microscopes allow us to record activity at single cell resolution throughout the brain of transgenic fish expressing genetically-encoded calcium indicators, which report neural activity with changes in fluorescence.

Our focus is on brain circuits that process visual stimuli (for example resembling predators or prey) to control adaptive behavioural responses (such as escape or hunting). We build virtual reality environments for zebrafish and examine neural activity underlying visual perception and the generation of locomotor programmes whilst the animal responds to naturalistic visual stimuli.

Publications

Publications

Hildebrand DGC, Cicconet M, Torres RM, Choi W, Quan TM, Moon J, Wetzel AW, Scott Champion A, Graham BJ, Randlett O, Plummer GS, Portugues R, Bianco IH, Saalfeld S, Baden AD, Lillaney K, Burns R, Vogelstein JT, Schier AF, Lee WA, Jeong WK, Lichtman JW, Engert F. Whole-brain serial-section electron microscopy in larval zebrafish. Nature (2017). doi:10.1038/nature22356

Zou M, Friedrich RW, Bianco IH. Targeted Electroporation in Embryonic, Larval, and Adult Zebrafish. Methods Mol Biol (2016). 1451:259-69.

Bianco IH and Engert F. Visuomotor transformations underlying hunting behavior in zebrafish. Current Biology (2015). 25:1-16.

Hüsken U, Stickney HL, Roussigne M, Beretta CA, Brinkmann I, Young RM, Bianco IH, Tsalavouta M, Zigman M, Hawkins TA, Wen L, Zhang B, Blader P, Lin S, Wilson SW, Carl M. Tcf7l2-dependent asymmetries in Wnt signalling mediate the left-right asymmetric differentiation of habenular neurons. Current Biology (2014). 24:2217-27.

Concha MC, Bianco IH, Wilson SW. Encoding asymmetry within neural circuits. Nature Reviews Neuroscience. (2012). 13:832-43.

Bianco IH, Ma L-H, Schoppik D, Robson DN, Orger MB, Beck JC, Li JM, Schier AF, Engert F, Baker R. The tangential nucleus controls a gravito-inertial vestibulo-ocular reflex. Current Biology (2012). 22:1285-95.

Bianco IH, Kampff AR, Engert F. Prey capture behavior evoked by simple visual stimuli in larval zebrafish. Front. Syst. Neurosci. (2011). 5:101.

Tawk M, Bianco IH, Clarke JD. Focal electroporation in zebrafish embryos and larvae. Methods Mol Biol. (2009). 546:145-51.

Roussigné M, Bianco IH, Wilson SW, Blader P. Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei. Development. (2009). 136:1549-57.

Bianco IH, Wilson SW. The habenular nuclei: a conserved asymmetric relay station in the vertebrate brain. Philos Trans R Soc Lond B Biol Sci. (2009). 364:1005-20.

Bianco IH, Carl M, Russell C, Clarke JD, Wilson SW. Brain asymmetry is encoded at the level of axon terminal morphology. Neural Dev. (2008). 3:9.

Carl M, Bianco IH, Bajoghli B, Aghaallaei N, Czerny T, Wilson SW. Wnt/Axin1/beta-catenin signaling regulates asymmetric nodal activation, elaboration, and concordance of CNS asymmetries. Neuron. (2007). 55:393-405.

Barth KA, Miklosi A, Watkins J, Bianco IH, Wilson SW, Andrew RJ. fsi zebrafish show concordant reversal of laterality of viscera, neuroanatomy, and a subset of behavioral responses. Curr Biol. (2005). 15:844-50.

Aizawa H, Bianco IH, Hamaoka T, Miyashita T, Uemura O, Concha ML, Russell C, Wilson SW, Okamoto H. Laterotopic representation of left-right information onto the dorso-ventral axis of a zebrafish midbrain target nucleus. Curr Biol. (2005). 15:238-43.

Mo E, Amin H, Bianco IH, Garthwaite J. Kinetics of a cellular nitric oxide/cGMP/phosphodiesterase-5 pathway. J Biol Chem. (2004). 279:26149-58.